Alla flessibilità progettuale e produttiva fanno seguito anche le illustri collaborazioni che la SOLTEC porta avanti con i principali Istituti ed Enti di Ricerca Italiani e Internazionali, come il recente studio condotto in collaborazione con il Dipartimento di Scienze Ambientali e di Farmacologia dell’Università di Milano. Utilizzando un’apparecchiatura della linea SONICA^® e un apposito detergente disinfettante, è stata infatti portata a termine un’importante ricerca sulla decontaminazione, detersione e disinfezione dello strumentario chirurgico.

Annals of Microbiology, 54 (2), 233-243 (2004)

A new methodology for decontamination of dental instruments by an ultrasonic cleaner based on Sweep System Technology

P. DI GENNARO¹, G. BESTETTI^1*, A. RADAELLI²,M. PAGANINI³, C. DE GIULI MORGHEN³, M. NERI³

¹Department of Environmental Sciences, University of Milano-Bicocca, 20126 Milano; ²Department of Pharmacological Sciences, ³Department of Medical Pharmacology,University of Milan, 20129 Milano, Italy

Received 20 January 2004 / Accepted 25 February 2004

Abstract

The efficiency of a decontamination procedure by sonication for different dental instruments after experimental microbial and viral contamination was tested. Both germicidal and virucidal activity of sonication in the presence or absence of a cationic biobiguanid disinfectant was assessed following three different disinfection/sterilisation protocols. Dental instruments were contaminated with a mixed culture of Enterococcus faecium, Staphylococcus sp., Pseudomonas aeruginosa, Mycobacterium sp., Escherichia coli and Bacillus subtilis, or with Poliovirus type 1 and Herpesvirus simplex type 1 (HHV-1), exposed to ultrasonic treatment in an ultrasonic bath and the surviving microorganisms titered. The results showed that an effective disinfection of dental instruments, expressed by an equal or higher than 4 logs microbial and viral reduction, can be obtained after 15 min or 10 min sonication in an ultrasonic cleaner equipped with a Sweep System Technology. Conversely, by the combined action of chemical disinfection and ultrasonic treatment in the same device, a sterilising effect was obtained after only 5 min for microbial and 10-15 min for virally contaminated instruments. The synergistic effect of chemical and physical means, as already accepted as an effective cleaning procedure of medical instruments, can therefore be applied to obtain a safe and effective sterilisation of dental instruments potentially contaminated by organic fluids and dental material harbouring pathogenic microbes and viruses.

Key words: disinfection, sterilisation, ultrasounds, dental instruments, ultrasonic cleaner.

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